Since today’s seminar speaker here is Dr. Daniel Voytas from Minnesota, I went looking for some of his recent papers. I came across this recent one, from him and his collaborators, reporting a series of vectors optimized for plant transgenesis with various types of CRISPR activities, including multi-plexed targeting and transcriptional activation. At a glance the experiments look convincing, and the vector series is available at Addgene. Folks in my lab who are interested in multi-plexed CRISPR/Cas9 targeting might want to look at this study.
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation. (2015; Plant Physiology. doi: 10.1104/pp.15.00636)
