Our multidisciplinary research group focuses on nano-micro-macro scale devices for biomedical applications. We develop and deploy innovative materials, devices and systems to enable next-generation medical diagnosis and life science research. We aim to address various challenges in sensitivity, specificity, high-throughput, multiplexing, and cost etc. We are committed to realizing these goals by applying micro and nano-technological towards medical applications.

The projects performed to achieving our over-arching goals are listed below.

I. Point-of-care nucleic acid testing (NAT)

Nucleic acid testing (NAT) is currently the most sensitive method available for identifying the infectious pathogens. Nevertheless, NAT-based diagnosis developed to date mostly require sophisticated infrastructures, reagents, and skilled technicians. While readily available in reference laboratories, NATs such as PCR remains inaccessible in resource-limited settings. Although extensive efforts have been undertaken towards point-of-care (POC) molecular diagnosis, a fully validated “sample-in-answer-out”  NAT system has not developed due to significant challenges of portability, sample preparation, and throughput. In response to this urgent need, we aim to develop low-cost field-deployable field-deployable NAT devices and systems, especially for infectious disease in resource-limiting areas. These NAT devices could be loaded with easily-obtainable raw biospecimen such as finger prick blood, making diagnostic testing faster and easier for identifying pathogens like Malaria, Zika, and HIV.

II. Nanofluidics: device physics, fabrication and application

Molecule/ion transport at the nanometer scale plays an important role in many biological, chemical, physical and engineering systems. Nanoscale channels offer a unique platform to explore new phenomena appearing for molecule confined in nanometers scales. New transport behavior and functionalities can be developed by taking advantage of the specific couplings occurring at these scales.  The major challenges in nanofluidic research are the lack of fundamental understanding of the dynamic electric field coupling to the charged molecules at the nanoscale and the lack of reliable and low-cost top-down fabrication methods. We aim to understand the device physics of nanofluidic devices, to explore viable device fabrication and integration technologies and applications.

III. Cell Mechanotyping

The mechanical behavior of individual cells plays an important role in regulating various biological activities at the molecular and cellular level. It can serve as a promising label-free marker of cells’ physiological states. In the past decades, several technologies have been developed for understanding the association between the cell mechanical changes and human diseases. However, numerous technical challenges still remain for realizing high-throughput, robust and easy-to-perform measurements on single-cell mechanical properties.