No samples today… Cleaning the columns… Boring. Maybe I will be Keratin for Halloween: fur coat, long hair, woolen gloves…
Running 4 samples and 4 blanks for Juan this afternoon (overnight), then cleaning before the 4 IP samples for Donna. John’s set will be next.
Orbi will be busy the next 3-4 days running two in-solution digests (8-hours each) for Tugba and Tania followed by 6 samples for John. More samples are coming later today!
Yesterday, Orbi quit mid-run, so the queue is now 1 day behind. Samples are running: fingers crossed! If the problem persists, Orbi will be down for 2 days.
Orbi is running 2 in-gel digested samples for Juan and 5 in-gel digested samples for Tania (yep, that’s me). Tugba’s sample is next…
Today is a gel day: running a couple of gels for myself and a customer and some in-gel digestions. Orbi will be running a TMT 2-plex sample in triplicate for Brian today (for 24 hours).
This morning, tuning and calibrating Orbi for the intact protein analysis (for Tyler and Edward). After that, back to nano-LC: 4 samples for Yaqi.
Cleaning and troubleshooting Orbi today. Filtering your samples is an easy way to get your samples analyzed without a delay.
Setting up 4 TMT-labeled samples in triplicate. Orbi will be working this weekend. ETA: B. Cereus TMT-labeled samples are running, 230 min/sample.
I get a lot of inquiries about our PAGE (polyacrylamide gel electrophoresis) equipment, so here are some information on the equipment we have and the supplies we use.
Cost: It’s free!
We share our PAGE equipment with you at no charge. You need to purchase your own supplies: e.g. gels, standards, buffers, and other consumables. Sometimes we have supplies left over from the Facility experiments, which we will gladly share with you as well. Please, ask before you buy your own!
Our power supply is a basic model; it does not store programmed methods. It can run up to 4 cells simultaneously, as long as the conditions are the same.
We have three Bio-Rad electrophoresis cells, a Mini-PROTEAN Tetra for up to 4 mini-gels (8.6 x 6.7 cm), a Criterion cell for up to 2 midi-gels (13.3 x 8.7 cm), and a Criterion Dodeca cell for up to 12 midi-gels. We also have a blotter for 13.3 x 8.7 cm gels.
2D PAGE, first dimension (isoelectric focusing, IEF)
Our PROTEAN IEF cell (Bio-Rad) is capable of storing methods, and accepts 7 cm, 11 cm, and 18 cm focusing trays. We also have rehydration trays for all three IPG (immobilized pH gradient) strip lengths.
Gel casting equipment
You can use our gel casting stands and glass plates (0.75 mm) to cast your own mini-gels. In my experience, 1-mm thick gels hold up much better, especially for low %T (4-10%). Bring your own plates if you wish!
The Pierce precast mini gels from Fisher are compatible with the Bio-Rad Mini-PROTEAN cell. We do not buy precast gels from Bio-Rad due to the high shipping costs. Fisher orders ship free. You can learn more here.
Immobilized pH gradient (IPG) strips are also available from Fisher (GE Healthcare Immobiline DryStrip gels, for example). The prices are comparable with the Bio-Rad’s, and the shipping is free.
05/28/2014 ETA: According to the GE Healthcare website, Immobiline IPG strips and buffers are not compatible with the Bio-Rad IEF cell running conditions. I am not familiar with the GE system and cannot tell you whether this is true; so to be safe, please use Bio-Rad IPG strips and buffers. If anyone has successfully run Immobiline strips on Bio-Rad IEF cell, please share with us! This information could save your colleagues both time and money!
Disclaimer: We do not receive any form of compensation for promoting any brand or a particular distributor.
Still have questions? Let’s meet and talk about your project!