Tag Archives: shared equipment

Tania

February 17, 2016

The Facility’s PAGE equipment is free to use. If you don’t know how to run a gel, Tania can help!

Professor Ozbolat's students, Kerim (left) and Madhuri (right) are using PAGE equipment with Donna's (center) guidance.

Professor Ozbolat’s students, Kerim (left) and Madhuri (right) are using PAGE equipment with Donna’s (center) guidance.

Plate reader

The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Please contact Tatiana to reserve the plate reader time: tnl1@psu.edu, 814-863-3503. There is currently no charge for using this plate reader.

The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 Multi-Mode Microplate Reader possesses the five modes preferred by the majority of our users providing the benefit of multiple detection modes in one platform:

UV-Visible Absorbance (Abs)

Fluorescence Intensity (FI)

Time-Resolved Fluorescence (TRF)

Fluorescence Polarization (FP)

Glow Luminescence (Lumi)

User Manual – SpectraMax M3 M4 M5 M5e

Software Manual – SoftMax Pro

1D and 2D PAGE equipment and supplies

I get a lot of inquiries about our PAGE (polyacrylamide gel electrophoresis) equipment, so here are some information on the equipment we have and the supplies we use.

Cost: It’s free!

We share our PAGE equipment with you at no charge. You need to purchase your own supplies: e.g. gels, standards, buffers, and other consumables. Sometimes we have supplies left over from the Facility experiments, which we will gladly share with you as well. Please, ask before you buy your own!

1D PAGE

Power supply

Our power supply is a basic model; it does not store programmed methods. It can run up to 4 cells simultaneously, as long as the conditions are the same.

Electrophoresis cells

We have three Bio-Rad electrophoresis cells, a Mini-PROTEAN Tetra for up to 4 mini-gels (8.6 x 6.7 cm), a Criterion cell for up to 2 midi-gels (13.3 x 8.7 cm), and a Criterion Dodeca cell for up to 12 midi-gels. We also have a blotter for 13.3 x 8.7 cm gels.

2D PAGE, first dimension (isoelectric focusing, IEF)

Our PROTEAN IEF cell (Bio-Rad) is capable of storing methods, and accepts 7 cm, 11 cm, and 18 cm focusing trays. We also have rehydration trays for all three IPG (immobilized pH gradient) strip lengths.

Gel casting equipment

You can use our gel casting stands and glass plates (0.75 mm) to cast your own mini-gels. In my experience, 1-mm thick gels hold up much better, especially for low %T (4-10%). Bring your own plates if you wish!

Supplies

The Pierce precast mini gels from Fisher are compatible with the Bio-Rad Mini-PROTEAN cell. We do not buy precast gels from Bio-Rad due to the high shipping costs. Fisher orders ship free. You can learn more here.

Immobilized pH gradient (IPG) strips are also available from Fisher (GE Healthcare Immobiline DryStrip gels, for example). The prices are comparable with the Bio-Rad’s, and the shipping is free.

05/28/2014 ETA: According to the GE Healthcare website, Immobiline IPG strips and buffers are not compatible with the Bio-Rad IEF cell running conditions. I am not familiar with the GE system and cannot tell you whether this is true; so to be safe, please use Bio-Rad IPG strips and buffers. If anyone has successfully run Immobiline strips on Bio-Rad IEF cell, please share with us! This information could save your colleagues both time and money!

Disclaimer: We do not receive any form of compensation for promoting any brand or a particular distributor.

Still have questions? Let’s meet and talk about your project!