Troubleshooting PCR reactions is ever so much fun! I’m discovering the difficulty of working with soil and working with different types of soil, then trying to isolate certain genes (DNA strands) from those different soils! The methods that others used for their water samples, or even soil samples, don’t necessarily work! So, for me to analyzing the antibiotic resistance genes of interest is requiring some trouble shooting, which means lots of different PCR runs and gels!

Not very good separation and bands are a little hard to see. Needed to run the gel a little longer.

Too many bands, but definitely nice run time on the gel to get those bands separated!

Looking better, but still not quite there yet!!!