Protocols

Research Protocols Used in the FRI

Protocols used in the FRI are made available on our Learning Management System, Canvas. To provide researchers with indefinite access to these resources we also provide them here!

Molecular Biology Protocols

  • DNA extraction
  • DNA quantification
  • Polymerase Chain Reaction (PCR)
  • Primer design for PCR-seq
  • Gel Electrophoresis
  • DNA purification for Sanger sequencing
  • Bioinformatic analysis of Sanger sequencing

Microbiology Protocols

  • Nutrient Agar Growth Media
  • Nutrient Agar Liquid Broth Media
  • Surface sampling
  • Plate swabbing
  • Colony Forming Unit (CFU) quantification
  • single colony isolation for liquid monoculture inoculation
  • Kirby-Bauer assay and analysis

General Lab Protocols

  • Lab safety
  • Wearing PPE
  • Disposing waste
  • Pipetting
  • Using a vortex
  • Using a bunsen burner
  • Using a thermoshaker
  • Using a centrifuge
  • Using a nanodrop
  • Using a thermocycler (PCR machine)
  • Using a gel electrophoresis rig and power source
  • Using a lab microwave (not for food)
  • Using a thermostatic bacterial growth incubator 
  • Using the autoclave

 

 

Does this page need to be updated? Email the FRI Coordinator: carly.sjogren@psu.edu.