02/21/2020: Dr. Hedglin visits The University of Kansas Medical Center to present a seminar on human DNA damage tolerance

Dr. Hedglin visited the University of Kansas Medical Center to present a seminar  on “Mechanistic Insights into Human DNA Damage Tolerance from Fluorescence-based Studies.” During the seminar, Dr. Hedglin presented data on a collaborative project with the lab of Dr. Tae-Hee Lee in the Department of Chemistry at PSU. Specifically, work from Dr. Mingjie Li… Read More 02/21/2020: Dr. Hedglin visits The University of Kansas Medical Center to present a seminar on human DNA damage tolerance

01/22/2020: The Murakami Lab, in collaboration with the Hedglin Lab, publishes an article in Nucleic Acids Research (NAR) on reiterative transcription

Reiterative transcription is a non-canonical form of RNA synthesis by RNA polymerase in which a ribonucleotide specified by a single base in the DNA template is repetitively added to the nascent RNA transcript. The Murakami lab previously determined the X-ray crystal structure of the bacterial RNA polymerase engaged in reiterative transcription from the pyrG promoter,… Read More 01/22/2020: The Murakami Lab, in collaboration with the Hedglin Lab, publishes an article in Nucleic Acids Research (NAR) on reiterative transcription

12/30/2019: Article on human DNA damage tolerance from the Hedglin Lab published in Biochemistry

Translesion DNA synthesis (TLS) bypasses DNA lesions encountered during S-phase and is critical for cell survival after exposure to DNA-damaging agents. In humans, Rad6/Rad18 attaches single ubiquitin moieties (i.e. monoubiquitination) to proliferating cell nuclear antigen (PCNA) sliding clamps encircling primer/template (P/T) junctions that are stalled at DNA lesions. TLS occurs via PCNA monoubiquitination-independent and -dependent… Read More 12/30/2019: Article on human DNA damage tolerance from the Hedglin Lab published in Biochemistry

01/30/2019: Article on human DNA damage tolerance from the Hedglin Lab published in The Journal of Biological Chemistry

DNA damage tolerance permits bypass of DNA lesions encountered during S-phase and may be carried out by translesion DNA synthesis (TLS). Human TLS requires selective monoubiquitination of proliferating cell nuclear antigen (PCNA) sliding clamps encircling damaged DNA.  This posttranslational modification (PTM) is catalyzed by Rad6/Rad18. Recent studies revealed that replication protein A (RPA), the major ssDNA-binding… Read More 01/30/2019: Article on human DNA damage tolerance from the Hedglin Lab published in The Journal of Biological Chemistry