Project Team
Students
Yasmitha Andra
Clinical Biotechnology
Harrisburg
Faculty Mentors
Sairam Rudrabhatla
Harrisburg
Biological Sciences
Shobha Rudrabhatla
Harrisburg
Biological Sciences
Project
https://sites.psu.edu/mcreu/files/formidable/2/2024-07-22/MC-REU-Yasmitha-A-PDF.pdf
Project Video
Project Abstract
This study explores the impact of various plant growth regulators (PGRs), specifically cytokinins and auxins, on various explants tested for organogenesis of industrial hemp (Cannabis sativa L.). Industrial hemp research is vital for carbon sequestration, GHG (greenhouse gas) reduction, and producing various end-user products such as hempcrete, ceiling tiles, biodegradable plastics, textiles, and various other secondary metabolites such as cannabinoids and terpenes. In this study, hemp seeds were sterilized and germinated under in vitro conditions on Murashige and Skoog (MS) medium. Young explants, such as leaves, hypocotyls, cotyledons, and axillary buds were transferred to a shooting medium containing 0.5 mg/L and 1.0 mg/L concentrations of the cytokinins thidiazuron (TDZ) and benzylaminopurine (BA). Results demonstrated from formation of shoots and roots after placing on indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA), at various concentrations, along with copper sulfate. We demonstrate here an optimized tissue culture protocol for organogenesis in industrial hemp.
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