What is TMT?

TMT (tandem mass tags) are labeling reagents for comparative mass-spectrometry-based proteomics, a Thermo equivalent of iTRAQ. Most popular TMT are amine reactive, but SH-reactive tags are also available. Each isobaric TMT reagent has an amine-reactive NHS-ester group, a spacer arm, and a tandem MS reporter. Either intact proteins or their tryptic digests can be labeled, and up to 6 experimental conditions can be compared in terms of protein expression differences. You can find more information about TMT reagents here.

Our facility is capable of performing both TMT and iTRAQ experiments.