According to the Praxair’s State College employee Jennifer at the extension 7003, it is perfectly normal; all you have to do is to connect the tank and start using the nitrogen. “Pressure builds up and the tank is venting”, Jennifer tells me, as if this was my first delivery of high-pressure LN2. (Translation: “Praxair got your money, now it’s your problem”.)
Normal liquid-to-gas conversion is about 2-3%, when not in use; the tank I received yesterday has already managed to convert about 50% of its 240 L to gas. When high-pressure cylinders are not being used, they will vent occasionally. Unlike other liquid nitrogen cylinders of the same make and model delivered by Praxair , this one suffers from incontinence: it has already lost half of its volume within 24 hours because it vents non-stop through a faulty valve. Thankfully, we have good ventilation, otherwise we’d suffocate.
What I expected from Praxair was an apology for delivering a defective product and an offer to replace it with a usable one. Instead, I got a childish explanation of the workings of a liquid nitrogen cylinder.
The serial number of the poor leaky guy is M699-009-GD (Part No. GL65-OC 14-EZ-MG M, Taylor-Wharton). If it shows up in my lab again, venting away – it goes straight back where it came from.
This is a friendly reminder that the plate reader is a shared instrument, and many researchers rely on it being maintained in good condition. While it is free for everyone to use, it is not free to repair and maintain. Please be courteous to your colleagues and take good care of the plate reader.
Please close the drawer, turn off the instrument and the computer, and cover the instrument before leaving.
Regrettably, I am unable to clean after facility visitors and check the plate reader area; and I thank everyone who helps to keep the plate reader area in my laboratory clean and tidy.
Yesterday, the reader was left turned on and open: not a good thing given the amount of dust and sand coming out of the AC ducts in the lab. (It is a very old building.)
Plate reader left turned on and open on July 31
Restoring the equipment back to operating conditions.
If you are interested in learning how proteins are identified using mass-spectrometry-based proteomics, Proteome Software website has an excellent collection of short and informative essays.
We have updated the Biotyper library which now contains 6903 entries. More than 80 new yeast MSPs and more than 400 each Gram positive and Gram negative bacterial MSPs were included in this update.
The Facility’s PAGE equipment is free to use. If you don’t know how to run a gel, Tania can help!
Professor Ozbolat’s students, Kerim (left) and Madhuri (right) are using PAGE equipment with Donna’s (center) guidance.