Category Archives: Facility news

Plate reader

The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Please contact Tatiana to reserve the plate reader time: tnl1@psu.edu, 814-863-3503. There is currently no charge for using this plate reader.

The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 Multi-Mode Microplate Reader possesses the five modes preferred by the majority of our users providing the benefit of multiple detection modes in one platform:

UV-Visible Absorbance (Abs)

Fluorescence Intensity (FI)

Time-Resolved Fluorescence (TRF)

Fluorescence Polarization (FP)

Glow Luminescence (Lumi)

User Manual – SpectraMax M3 M4 M5 M5e

Software Manual – SoftMax Pro

New use rates

As the number of Facility customers grows, the turnaround times are getting longer. The two major factors that slow us down are sample preparation and data interpretation.

The time spent on preparing and cleaning your samples is the time taken away from MS-related activities, e.g. operating and maintaining the equipment, processing and interpreting data, and developing better methods for your samples. Sample prep is something we can delegate to you, and many of our customers have been submitting MS-ready samples and getting their results quickly.

The new use rates are designed to save you money and time should you decide to prepare your own samples. This does not mean that we will no longer prepare your samples; it simply means that you will be billed for the staff time in addition to the instrument time if we have to spend a significant amount of time working on your samples.

To help you overcome beginner’s fright, I can present a series of 1-hr hands-on sample preparation workshops, which will be offered as needed (i.e. as soon as 5 people express interest in the same workshop topic). The cost will be $57 per person, and the protocols will be freely available on-line before the workshop announcement so that you can decide whether you wish to attend. I am open to suggestions for the first workshop, e-mail me at tnl1@psu.edu with the subject line ‘workshop topic’.

Gel imaging equipment give-away

We are giving away a set of gel-imaging equipment. There has been a very small number of requests for use of this equipment even though we offer this service at no charge. We would like to find a good home for this equipment – hopefully a shared facility – as we are making room for a new mass spectrometer.

1. Proteome Works spot cutter with fluorescent enclosure (Bio-Rad)

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2. GS-800 calibrated densitometer (Bio-Rad)

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3. Molecular imager FX Pro Plus laser scanner (Bio-Rad)

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4. PDQuest server (Bio-Rad)

Please feel free to stop by and take a look!

Tania

December 11, 2013

Orbi is running samples for Eric tonight. Eric is a student at Hershey.

M@LDI LR (aka Matilda) has left the facility today to join other Matildas of her age.

The Winter Holiday schedule is up on our door: we will be closed from Monday, December 23rd, through Friday, January 3rd, and back to work on January 6th. Please plan accordingly!

Highlights from today’s seminar

Direct analysis of TLC spots by MALDI-TOF

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“Edmass” sequencing, N-terminal and C-terminal sequencing of intact SOD1 protein

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RNA oligomer (28-mer) MW and sequence analysis – on just 1 microliter of sample

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PolyTools software screenshot

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Thank you all for participating and asking questions!