I can’t believe it’s been 2 weeks since the last post! We’ve been so busy! Today we are recovering from one of our electrical shutdowns: Orbi is baking out, MALDI is ready and is being used by Krista from the Meredith group.
Here’s the current Orbi queue: Katsu, Nick, Huihun, Joana, Jung (49 hours). MALDI queue: Alex, Jason, Core Facility standards (3 hours).
If Orbi behaves, all analyses should be done by Tuesday next week.
What a happy Friday! Orbi has finished Gaozhong’s samples and is running John’s gel bands. After a nice weekend-long wash, Orbi will be running 15 various IP samples (for Scott, Kenny, and Joana).
Thanks to the OPP electricians, we have our power restored, and the instruments are pumping down. Orbi will take some time to reach operating conditions, as usual. MALDI is ready for your samples. LCT and QTRAP should be ready tomorrow.
Meanwhile, Scott’s, Joana’s, and John’s gels are incubating with trypsin overnight.
Orbi is running Olga’s second sample, and Ninad’s two samples are next in the queue. Gaozhong’s, Ming-Yang’s, and Yang’s samples have been analyzed and the data will be processed on Monday.
I have analyzed Alex’s oligos using MALDI TOF, next will be polymers for Brooks on Monday.
Orbi was out of calibration again, so I had to recalibrate everything; it looks much better now. All gel bands have been extracted and are running now. Tomorrow, will be the negative mode day: Alex’s oligos (provided I can calibrate the Orbi).
MALDI is bored.
The instrument was not performing to the specs during the past 2 weeks, but it looks like the issue has been resolved with the help of two Thermo tech support specialists, Mike Henry and Mohit Jain. I will check the calibrations next week to make sure everything is to the specs.
Meanwhile, Orbi is analyzing Yuning’s digest. Ninad’s samples are next. I have cleaned all gel bands submitted to date, and they are currently incubating with trypsin.
MALDI-TOF is working. I ran polymer samples for Brooks. Next are oligonucleotides for Alex and milk samples for Asha.
It’s nice to be back! Orbi is pumping down after a 2-week break. I will calibrate it as soon as the temperature stabilizes – and we will be back online! There are a few samples in the queue, but they can be run fairly quickly.
MALDI TOF survived its first holiday break in Althouse and will be running a Biotyper plate and a few different samples (oligonucleotides, proteins, gel bands) today and tomorrow.
Orbi is running second set of Scott’s samples; the first set has been analyzed and the data processed. Next in the queue are Eric’s and Josh’s samples, after which the instrument will be shut down for the holidays.
MALDI will also be shut down tomorrow to avoid potential problems (based on the last year’s holidays experience).
Today is Monday. Tomorrow is safety inspection. I am extracting 48 gel slices.
On the bright side, Yuanjun’s data is crunching and should be ready by tomorrow, and the lights on all our boxes are green!
Orbi is running Ninad’s IPs. Eric’s samples are next. Scott’s gel lanes will be ready for proteolysis by tomorrow morning. We have heating, cooling, and electricity, all at the same time!
MALDI is experiencing severe deficit of your attention.