Our laboratory has developed a variety of carbon-detection NMR pulse programs. We have also modified many other pulse programs published by other laboratories so that they will work efficiently on our proton inner-coil TCI cryoprobe. The links below provide access to a UNIX tar file containing Bruker Avance language code for the pulse program, a parameter file for execution of the pulse program, and a pdf containing useful information. The parameter files were generated on our 500 MHz spectrometer, which has an Avance-III console and is running Topspin 2. Curation services are provided through Penn State’s ScholarSphere.
Basic 2D Carbon-Detected NMR
Bastidas, M., Gibbs, E.B., Sahu, D., & Showalter, S.A. (2015) “A Primer for Carbon-Detected NMR Applications to Intrinsically Disordered Proteins in Solution.” Con. Mag. Reson. A, 44A, 54-66 . (preprint_pdf)
(HN-Start)CON-IPAP (file download)
(HACA)CON-IPAP (file download)
3D Carbon-Detected NMR for Chemical Shift Assignment
O’Hare, B., Benesi, A.J., & Showalter, S.A. (2009) “Incorporating 1H-Chemical Shift Determination into 13C-Direct Detected Spectroscopy of Intrinsically Disordered Proteins in Solution.” J. Mag. Reson., 200, 354-358. (pdf)
H(CC)CO-IPAP (file download)
H(CC)CON-IPAP (file download)
Sahu, D., Bastidas, M., & Showalter, S.A. (2014) “Generating NMR Chemical Shift Assignments of Intrinsically Disordered Proteins Using Carbon-Detect NMR Methods.” Anal. Biochem., 449, 17-25. PMCID: PMC3944900 (pdf)
N(CA)CON-IPAP (file download)
N(CA)NCO-IPAP (file download)
Cook EC, Usher GA, Showalter SA. The Use of (13)C Direct-Detect NMR to Characterize Flexible and Disordered Proteins. Methods Enzymol. 2018; 611: 81-100. PubMed PMID: 30471706
(HACA)N(CA)CON-IPAP (file download)
(HACA)N(CA)NCO-IPAP (file download)
Carbon-Detected Amino Acid Specific (CAS-NMR)
Sahu, D., Bastidas, M., & Showalter, S.A. (2014) “Generating NMR Chemical Shift Assignments of Intrinsically Disordered Proteins Using Carbon-Detect NMR Methods.” Anal. Biochem., 449, 17-25. PMCID: PMC3944900 (pdf)
CAS-CACON-ALA (file download)
CAS-CANCO-ALA (file download)
CAS-CANCO-ASP (file download)
CAS-CACON-GLU (file download)
CAS-CANCO-GLU (file download)
CAS-CACON-GLY (file download)
CAS-CANCO-GLY (file download)
CAS-CACON-LA (file download)
CAS-CANCO-LA (file download)
CAS-CACON-SER (file download)
CAS-CANCO-SER (file download)
CAS-CACON-TAVI (file download)
CAS-CANCO-TAVI (file download)
CAS-CACON-THR (file download)
CAS-CANCO-THR (file download)
Spin Relaxation
Lawrence, C.W., & Showalter, S.A. (2012) “Carbon-Detected 15N NMR Spin Relaxation of an Intrinsically Disordered Protein: FCP1 Dynamics Unbound and in Complex with RAP74.” J. Phys. Chem. Lett., 3, 1409-1413. (pdf)
CON(T1)-IPAP (file download)
CON(T2)-IPAP (file download)
Residual Dipolar Couplings
Cook, E.C., Sahu, D., Bastidas, M., & Showalter, S.A. (2019) “Solution Ensemble of the C-Terminal Domain from the Transcription Factor Pdx1 Resembles an Excluded Volume Polymer.” J. Phys. Chem. B, 123, 106-116.
(HN)CON-JHN-IPAP (file download)
(HACA)CON-JNCO-IPAP (file download)
(HACA)CON-JCAHA-IPAP (file download)
(HACA)CON-JCACO-IPAP (file download)
These pulse programs and accompanying parameter sets provide solution NMR experiments for use in standard methods of analyzing isotopically enriched samples. These pulse programs and parameter files are written and tested for Bruker NMR spectrometers using Topspin 3.2, with optimized parameters for CryoProbes. The parameter files were saved on a spectrometer operating at 500 MHz proton resonance frequency. The pulse programs and parameter sets are provided “as is.” If used incorrectly, these programs may potentially damage your instrument. Users employ the pulse programs at their own risk. Neither Scott A. Showalter nor the Pennsylvania State University is liable for any physical or other damage incurred during the use of the pulse programs.