We uses a variety of strategies to understand the mechanism of gene regulation by chromatin structure at different levels. Currently, we are working on two main projects: (1) to identify and characterize factors that can lead to nucleosome depletion and (2) to mechanistically dissect long-distance chromosomal interactions that regulate gene expression. We measure gene expression in single live cells to probe how these chromatin features affect gene expression in terms of the average level, cell-to-cell variability (noise), and dynamics. We are using budding yeast as our primary model system, but we are venturing into the mammalian cells as well. Method-wise, we are using a combination of imaging, genetics, genomics, and computational methods. We are also developing new genetics and genomics tools for the projects above.