Fuel Cell
In the fuel cell the hydrogen produced by a bacterial culture reacts with oxygen from air producing electricity and water.
- Type: Solid Polymer Electrolyte (PEM), catalog # 3506 (Hydrogen & Fuel Cell,Claremont, CA).
- Power output: 0.6 W.
Operation of a PEM Fuel Cell
(Taken from fueleconomy.gov/feg/fcv_pem.shtml)
Production of Bio-Hydrogen
An overnight culture (50 mL) of E. coli BW25113 hyaB hybC hycA fdoG/pCA24N-fhlA (1) in complex-formate (100 mM) medium was used to inoculate 175 mL of fresh complex-formate (100 mM) medium; the culture was sparged with nitrogen, to remove oxygen, and incubated under anaerobic condition for six hours at 37°C.
Fan
A fan with an electric load of 20 mW was connected to the output of the fuel cell. Catalog #7306 (Hydrogen & Fuel Cell,Claremont, CA).
Reference
- “Metabolic Engineering to Enhance Bacterial Hydrogen Production,” T. Maeda, V. Sanchez-Torres, and T. K. Wood, Microb. Biotechnol. 1: 30-39 (2008).