Since I get asked often but the official workflow is not made available in our iLab portal yet, please find below description handy to plan your 10X Genomics’s single-cell RNA-seq projects!
The library prep cost can be found in the iLab:
10x Genomics Library Prep
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initiate request
$1,713.00 (Internal) |
For RNA-seq we sequence 20K reads per sample for up to 10K cells per the prep, so ~200M reads by NovaSeq 100 cycles will also need to be requested.
NovaSeq_SP ( 100 cycles – SR100, PE 2×50 ) (per flow cell)
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initiate request
$2,751.38 (Internal) |
is the smallest flowcell you will likely be using and it can hold ~750M reads. Even you occupy 400M reads, we will have to find someone else to fill up the rest of 350M. Depending on how soon you would like the sequence goes, you may have to pay more $ to fill up the flow cell.
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