My experience in detecting genomic variations using BioRad’s droplet digital PCR got featured in BioRadiation journal.
The title of my article is:
Validation of Allele-Specific Expression Predicted by RNA-Seq in Human Brain Specimens
and I discuss about how you can make good use of digital PCR (especially Bio-Rad’s QX100 ddPCR system! 🙂 I used ddPCR to accurately and directly quantify ASE (allele specific expression, a.k.a. allelic imbalance) after I struggled quite a bit from not getting convincing data by using real-time PCR. The costs and time constraints of ddPCR are negligible compared to other known methods, such as the SNaPshot multiplex system, for accurate ASE detection.
I have also successfully detected gene expression in single cell w/o any pre-amplification. When it comes to sensitivity and accuracy, ddPCR is the way to go! 🙂
-Yuka