The publications below are the joint publications of the Bollinger/Krebs group. Click here for complete publication lists of Marty Bollinger and Carsten Krebs.
2024
Burke, E. J.; Copeland, R. A.; Dixit, Y.; Krebs, C.; Bollinger, J. M., Jr. “Steric Perturbation of the Grob-like Final Step of Ethylene-Forming Enzyme Enables 3-Hydroxypropionate and Propylene Production” J. Am. Chem. Soc. 2024, 146, 1977-1983. [link to publisher’s website]
2023
Slater, J. W.; Lin, C.-Y.; Neugebauer, M. E.; McBride, M. J.; Sil, D.; Nair, M. A.; Katch, B. J.; Boal, A. K.; Chang, M. C. Y.; Silakov, A.; Krebs, C.; Bollinger, J. M., Jr. “Synergistic Binding of the Halide and Cationic Prime Substrate of l-Lysine 4-Chlorinase, BesD, in Both Ferrous and Ferryl States” Biochemistry 2023 62, 2480–2491. [link to publisher’s website]
Maio, N.; Raza, Md. K.; Li, Y.; Zhang, D.-L.; Bollinger, J. M., Jr.; Krebs, C.; Rouault, T. A. “An Iron–Sulfur Cluster in the Zinc-Binding Domain of the SARS-CoV-2 Helicase Modulates its RNA- Binding and -Unwinding Activities” Proc. Natl. Acad. Sci. USA 2023, 120, e2303860120. [link to publisher’s website]
Manesis, A. C.; Slater, J. W.; Cantave, K.; Bollinger, J. M., Jr.; Krebs, C.; Rosenzweig, A. C. “Capturing a bis-Fe(IV) State in Methylosinus trichosporium OB3b MbnH” Biochemistry 2023 62, 1082-1092. [link to publisher’s website]
McBride, M. J.; Pope, S. M.; Nair, M. A.; Sil, D.; Salas-Sola, X. E.; Krebs, C.; Bollinger, J. M., Jr.; Boal, A. K. “Methods for Biophysical Characterization of SznF, a Member of the Heme-Oxygenase-like Diiron Oxidase/Oxygenase Superfamily” in “Oxygen Sensing: Methods in Molecular Biology” ed Weinert, E. – Springer, New York, 2023 Chapter 9, 123-154. [link to publisher’s website]
2022
Lin, C.-Y.; Muñoz Hernández, A. L.; Laremore, T. N.; Silakov, A.; Krebs, C.; Boal, A. K.; Bollinger, J. M., Jr. “Use of Non-canonical Tyrosine Analogs to Probe Control of Radical Intermediates during Endoperoxide Installation by Verruculogen Synthase (FtmOx1)” ACS Catalysis 2022 12, 6968-6979. [link to publisher’s website]
Panth, N.; Wenger, E. S.; Krebs, C.; Bollinger, J. M., Jr., Grossman, R. B. “Synthesis of 6,6- and 7,7-difluoro-1-acetamidopyrrolizidines and their oxidation catalyzed by the nonheme Fe oxygenase LolO” ChemBiochem 2022 23, e202200081 . [link to publisher’s website]
McBride, M. J.; Nair, M.; Sil, D.; Slater, J. W.; Neugebauer, M. E.; Chang, M. C. Y.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr. “A Substrate-triggered μ-Peroxodiiron(III) Intermediate in the 4-Chloro-L-Lysine-Fragmenting Heme-Oxygenase-like Diiron Oxidase (HDO) BesC: Substrate Dissociation from, and C4 Targeting by, the Intermediate” Biochemistry 2022, 61, 689–702. [link to publisher’s website]
Park, Y. J.; Jodts, R. J.; Slater, J. W.; Reyes, R. M.; Winton, V. J.; Montaser, R. A.; Thomas, P. M.; Dowdle, W. B.; Ruiz, A.; Kelleher, N. L.; Bollinger, J. M., Jr.; Krebs, C.; Hoffman, B. M., Rosenzweig, A. C. “A Mixed-Valent Fe(II)Fe(III) Species Converts Cysteine to an Oxazolone/Thioamide Pair in Methanobactin Biosynthesis” Proc. Acad. Natl. Sci. USA 2022, 119, e2123566119. [link to publisher’s website]
2021
Copeland, R. A.; Zhou, S.; Schaperdoth, I.; Shoda, T. K. C.; Bollinger, J. M., Jr., Krebs, C. “Hybrid Radical-Polar Pathway for Excision of Ethylene from 2-Oxoglutarate by an Iron Oxygenase” Science 2021, 373, 1489-1493. [link to publisher’s website]
Maio, N.; Lafont, B. A. P.; Sil, D.; Li, Y.; Bollinger, J. M., Jr.; Krebs, C.; Pierson, T. C.; Lineham, W. M. Rouault, T. A. “Fe-S Cofactors in the SARS-CoV-2 RNA-Dependent RNA Polymerase are Potential Antiviral Targets” Science 2021, 373, 236-241. [link to publisher’s website]
Copeland, R. A.; Davis, K. M.; Shoda, T. K. C.; Blaesi, E. J.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr. “An Iron(IV)-oxo Intermediate Initiating L-Arginine Oxidation but not Ethylene Production by the 2-Oxoglutarate-dependent Oxygenase, Ethylene-Forming Enzyme (EFE)” J. Am. Chem. Soc. 2021, 143, 2293-2303. [link to publisher’s website]
2020
Liu, G.; Sil, D.; Tong, W.-H.; Maio, N.; Bollinger, J. M., Jr.; Krebs, C.; Rouault, T. A.
“Dependence of Heme Biosynthesis on Iron Sulfur Cluster Biogenesis: Human Aminolevulinic Acid Dehydratase is an Unrecognized Iron-Sulfur Protein” Nature Communications 2020, 11, 6310-6320. [link to publisher’s website]
Srnec, M.; Iyer, S. R.; Dassama, L. M. K.; Park, K.; Wong, S. D.; Sutherlin, K. D.; Yoda, Y.; Kobayashi, Y.; Kurokuzu, M.; Saito, M.; Seto, M.; Krebs, C.; Bollinger, J. M., Jr.; Solomon, E. I. “NRVS Definition of the Facial Triad FeIV=O Intermediate in Taurine Dioxygenase: Evaluation of Structural Contributions to Hydrogen Atom Abstraction” J. Am. Chem. Soc. 2020, 142, 18886-18896. [link to publisher’s website]
Rajakovich, L. J.; Zhang, B.; McBride, M. J., Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr. “Emerging Structural and Functional Diversity in Proteins with Dioxygen-Reactive Dinuclear Transition Metal Cofactors” in “Comprehensive Natural Products III: Chemistry and Biology” edited by Liu, H.-w. and Begley, T. P., Elsevier, Amsterdam, 202
0. [link to publisher’s website]
McBride, M. J.; Sil, D.; Ng, T. L.; Crooke, A M.; Kenney, G. E.; Tysoe, C. R.; Zhang, B.; Balskus, E. P.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr.
“A Peroxodiiron(III) Intermediate Mediating both N-Hydroxylation Steps in Biosynthesis of the N-Nitrosourea Pharmacophore of Streptozotocin by SznF” J. Am. Chem. Soc. 2020, 142, 11818-11828. [link to publisher’s website]
Cutsail, G. E., III; Blaesi, E. J.; Pollock, C. J.; Bollinger, J. M., Jr.; Krebs, C.; DeBeer, S. “High-Resolution Iron X-ray Absorption Spectroscopic and Computational Studies of Non-Heme Diiron Peroxo Intermediates” J. Inorg. Biochem. 2020, 203, 110877. [link to publisher’s website]
2019
Zhou, S.; Pan, J.; Davis, K. M.; Schaperdoth, I.; Wang, B.; Boal,, A. K.; Krebs, C.; Bollinger, J. M., Jr. “Steric Enforcement of cis-Epoxide Formation in the Radical C–O-Coupling Reaction by which (S)-2-Hydroxypropylphosphonate Epoxidase (HppE) Produces Fosfomycin” J. Am. Chem. Soc. 2019, 141, 51, 20397-20406. [link to publisher’s website]
Davis, K. M.; Altmyer, M.; Martinie, R. J.; Schaperdoth, I.; Krebs, C.; Bollinger, J. M., Jr.; Boal, A. K. “Structure of a Ferryl Mimic in the Archetypal Iron(II)- and 2-(Oxo)-Glutarate-Dependent Dioxygenase, TauD” Biochemistry 2019, 58, 4218-4223. [link to publisher’s website]
Pan, J.; Wenger, E. S.; Matthews, M. L.; Pollock, C. J.; Bhardwaj, M.; Kim, A. J., Allen, B. D.; Grossman, R. B.; Krebs, C.; Bollinger, J. M., Jr. “Evidence for Modulation of Oxygen-Rebound Rate in Control of Outcome by Iron(II)- and 2-Oxoglutarate-Dependent Oxygenases” J. Am. Chem. Soc. 2019, 141, 15153-15165. [link to publisher’s website]
Zhang, B.; Rajakovich, L. J.; Van Cura, D.; Blaesi, E. J.; Mitchell, A. J.; Tysoe, C. R.; Zhu, X.; Streit, B. R.; Rui, Z.; Zhang, W.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr. “Substrate-triggered Formation of a Peroxo-Fe2(III/III) Intermediate during Fatty Acid Decarboxylation by UndA” J. Am. Chem. Soc. 2019, 141, 14510-14514. [link to publisher’s website]
Chekan, J. R.; Ongpipattanakul, C.; Wright, T. R.; Zhang, B.; Bollinger, J. M., Jr.; Rajakovich, L. J.; Krebs, C.; Cicchillo, R. M.; Nair, S. K. “Molecular Basis for Enantioselective Herbicide Degradation Imparted by Aryloxyalkanoate Dioxygenases in Transgenic Plants” Proc. Natl. Acad. Sci., U.S.A., 2019, 116, 13299-13304. [link to publisher’s website]
Dunham, N. P.; Del Río Pantoja, J. M.; Zhang, B.; Rajakovich, L. J.; Allen, B. D.; Krebs, C.; Boal, A. K.; Bollinger, J. M., Jr. “Hydrogen Donation but not Abstraction by a Tyrosine (Y68) During Endoperoxide Installation by Verruculogen Synthase (FtmOx1)” J. Am. Chem. Soc. 2019, 141, 9964-9979. [link to publisher’s website]
Rajakovich, L. J.; Pandelia, M.-E.; Mitchell, A. J.; Chang, W.-c.; Zhang, B.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr. “A New Microbial Pathway for Organophosphonate Degradation Catalyzed by Two Previously Misannotated Non-Heme-Iron Oxygenases” Biochemistry 2019, 58, 1627-1647. [link to publisher’s website]
2018
Dunham, N. P.; Mitchell, A. J.; Del Río Pantoja, J. M.; Krebs, C.; Bollinger, J. M., Jr.; Boal, A. K. “α-Amine Desaturation of D-Arginine by the Iron(II)- and 2-(Oxo)-Glutarate-Dependent L-Arginine 3-Hydroxylase, VioC” Biochemistry 2018, 57, 6479–6488. [link to publisher’s website]
Blaesi, E. J.; Palowitch, G. M.; Hu, K.; Kim, A. J.; Rose, H. R.; Alapati, R.; Lougee, M. G.; Kim, H. J.; Taguchi, A. T.; Tan, K. O.; Laremore, T. N.; Griffin, R. G.; Krebs, C.; Matthews, M. L.; Silakov, A.; Bollinger, J. M., Jr.; Allen, B. D.; Boal, A. K. “Metal-Free Class Ie Ribonucleotide Reductase from Pathogens Initiates Catalysis with a Tyrosine-Derived Dihydroxyphenylalanine Radical” Proc. Natl. Acad. Sci., U.S.A., 2018, 115, 10022-10027. [link to publisher’s website]
Martinie, R. J.; Blaesi, E. J.; Bollinger, J. M., Jr.; Krebs, C.; Finkelstein, K. D.; Pollock, C. J. “Two-Color Valence-to-Core X-ray Emission Spectroscopy Tracks Cofactor Protonation State in a Class I Ribonucleotide Reductase” Angew. Chem., Int. Ed. 2018, 57, 12754-12758. [link to publisher’s website]
Dunham, N. P.; Chang, W.-c.; Mitchell, A. J.; Martinie, R. J.; Zhang, B.; Bergman, J. A.; Rajakovich, L. J.; Wang, B.; Silakov, A.; Krebs, C.; Boal, A. K.; Bollinger, J. M., Jr. “Two Distinct Mechanisms for C-C Desaturation by Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenases: Importance of alpha-Heteroatom Assistance” J. Am. Chem. Soc. 2018, 140, 7116–7126. [link to publisher’s website]
Rose, H. R.; Ghosh, M. K.; Maggiolo, A. O.; Pollock, C. J.; Blaesi, E. J.; Hajj, V.; Wei, Y.; Rajakovich, L. J.; Chang, W.-c.; Han, Y.; Hajj, M.; Krebs, C.; Silakov, A.; Pandelia, M.-E.; Bollinger, J. M., Jr.; Boal, A. K. “Structural Basis for Superoxide Activation of Flavobacterium johnsoniae Class I Ribonucleotide Reductase and for Radical Initiation by Its Dimanganese Cofactor” Biochemistry 2018, 57, 1372-1383. [link to publisher’s website]
Pan, J.; Bhardwaj, M.; Zhang, B.; Chang, W.-c.; Schardl, C. L.; Krebs C.; Grossman, R. B.; Bollinger, J. M., Jr. “Installation of the Ether Bridge of Lolines by the Iron- and 2-Oxoglutarate-Dependent Oxygenase, LolO: Regio- and Stereochemistry of Sequential Hydroxylation and Oxacyclization Reactions” Biochemistry 2018, 57, 2074-2083. [link to publisher’s website]
Kenney, G. E.; Dassama, L. M. K.; Pandelia, M.-E.; Gizzi, A. S.; Martinie, R. J.; Gao, P.; DeHart, C. J.; Schachner, L. F.; Skinner, O. S.; Ro, S. Y.; Zhu, X.; Sadek, M.; Thomas, P. M.; Almo, S. C.; Bollinger, J. M., Jr.; Krebs, C.; Kelleher, N. L.; Rosenzweig, A. C. “The Biosynthesis of Methanobactin” Science 2018, 359, 1411-1416. [link to publisher’s website]
2017
Martinie, R. J.; Pollock, C. J.; Matthews, M. L.; Bollinger, J. M., Jr.; Krebs, C.; Silakov, A. “Vanadyl as a Stable Structural Mimic of Reactive Ferryl Intermediates in Mononuclear Nonheme-Iron Enzymes” Inorg. Chem. 2017, 56, 13382–13389. [link to publisher’s website]
Mitchell, A. J.; Dunham, N. P.; Martinie, R. J.; Bergman, J. A.; Pollock, C. J.; Hu, K.; Allen, B. D.; Chang, W.-c.; Silakov, A.; Bollinger, J. M., Jr.; Krebs, C.; Boal, A. K. “Visualizing the Reaction Cycle in an Iron(II)- and 2-(Oxo)-Glutarate-Dependent Hydroxylase” J. Am. Chem. Soc. 2017, 139, 13830–13836. [link to publisher’s website]
Park, K.; Li, N.; Kwak, Y.; Srnec, M.; Bell, Caleb B., III ; Liu, L. V.; Wong, S. D.; Yoda, Y.; Kitao, S.; Seto, M.; Hu, M.; Zhao, J.; Krebs, C.; Bollinger, J. M., Jr.; Solomon, E. I. “Peroxide Activation for Electrophilic Reactivity by the Binuclear Non-heme Iron Enzyme AurF” J. Am. Chem. Soc. 2017, 139, 7062-7070. [link to publisher’s website]
Fuller, F. D.; Gul, S.; Chatterjee, R.; Burgie E. S.; Young, I. D.; Lebrette, H.; Srinivas, V; Brewster, A. S.; Michels-Clark, T.; Clinger, J.; Andi, B.; Ibrahim, M.; Pastor, E.; de Lichtenberg, C.; Hussein, R.; Pollock, C. J.; Zhang, M.; Stan, C. A.; Kroll, T.; Fransson, T.; Weninger, C.; Kubin, M.; Aller, P.; Lassalle, L.; Bräuer, P.; Amin, M.; Koroidov, S.; Roessler, C. G.; Allaire, M.; Sierra, R. G.; Docker, P. T.; Glownia, J. M.; Nelson, S.; Koglin, J.; Zhu, D.; Chollet, M.; Song, S.; Lemke, H.; Liang, M.; Sokaras, D.; Alonso-Mori, R.; Zouni, A.; Messinger, J.; Bergmann, U.; Boal, A. K.; Bollinger, J. M., Jr.; Krebs, C.; Högbom, M.; Philips, G. N, Jr.; Vierstra, R. D.; Sauter, N. K.; Orville, A. M.; Kern, J.; Yachandra, V. K.; Yano, J. “Drop-on-Demand Sample Delivery for Studying Biocatalysts in Action at XFELs” Nature Methods 2017, 14, 443-449. [link to publisher’s website]
Peck, S. C., Wang, C.; Dassama, L. M. K.; Zhang, B.; Guo, Y.; Rajakovich, L. J.; Bollinger, J. M., Jr.; Krebs, C.; van der Donk, W. A. “O-H Activation by an Unexpected Ferryl Intermediate during Catalysis by 2-Hydroxyethylphosphonate Dioxygenase” J. Am. Chem. Soc. 2017, 139, 2045-2052. [link to publisher’s website]
Martinie, R. J.; Blaesi, E. J.; Krebs, C.; Bollinger, J. M., Jr.; Silakov, A.; Pollock, C. J. “Evidence for a Di-µ-oxo Diamond Core in the Mn(IV)/Fe(IV) Activation Intermediate of Ribonucleotide Reductase from Chlamydia trachomatis” J. Am. Chem. Soc. 2017, 139, 1950-1957. [link to publisher’s website]
2016
Tamanaha, E.; Zhang, B.; Guo, Y.; Chang, W.-c.; Barr, E. W.; Xing, G.; St.Clair, J.; Ye, S.; Neese, F.; Bollinger, J. M., Jr.; Krebs, C. “Spectroscopic evidence for the two C-H-cleaving intermediates of Aspergillus nidulans isopenicillin N synthase” J. Am. Chem. Soc. 2016, 138, 8862-8874. [link to publisher’s website]
Srnec, M.; Wong, S. D.; Matthews, M. L.; Krebs, C.; Bollinger, J. M., Jr.; Solomon, E. I. “Electronic Structure of the Ferryl Intermediate in the α-Ketoglutarate Dependent Non-Heme Iron Halogenase SyrB2: Contributions to H Atom Abstraction Reactivity” J. Am. Chem. Soc. 2016, 138, 5110-5122. [link to publisher’s website]
2015
Rajakovich, L. J.; Nørgaard, H.; Warui, D. M.; Chang, W.-c.; Booker, S. J.; Krebs, C.; Bollinger, J. M., Jr.; Pandelia, M.-E. “Rapid Reduction of the Diferric-Peroxyhemiacetal Intermediate in Aldehyde-Deformylating Oxygenase by a Cyanobacterial Ferredoxin: Evidence for a Free-Radical Mechanism” J. Am. Chem. Soc. 2015, 137, 11695-11709. [link to publisher’s website]
Livada, J.; Martinie, R. J.; Dassama, L. M. K.; Krebs, C.; Bollinger, J. M., Jr.; Silakov, A. “Direct Measurement of the Radical Translocation Distance in the Class I Ribonucleotide Reductase from Chlamydia trachomatis” J. Phys. Chem. B 2015, 119, 13777-13784. [link to publisher’s website]
Martinie, R. J.; Livada, J.; Chang, W.-c.; Green, M. T.; Krebs, C.; Bollinger, J. M., Jr.; Silakov, A. “Experimental Correlation of Substrate Position with Reaction Outcome in the Aliphatic Halogenase, SyrB2” J. Am. Chem. Soc. 2015, 137, 6912-6919. [link to publisher’s website]
Bollinger, J. M., Jr.; Chang, W.-c.; Matthews, M. L.; Martinie, R. J.; Boal, A. K.; Krebs, C. “Mechanisms of 2-Oxoglutarate-Dependent Oxygenases: The Hydroxylation Paradigm and Beyond” in “2-Oxoglutarate-Dependent Oxygenases” edited by Hausinger, R. P. and Schofield, C. J.; The Royal Society of Chemistry, London, 2015, 95-122. [link to publisher’s website]
Warui, D. M.; Pandelia, M.-E.; Rajakovich, L. J.; Krebs, C.; Bollinger, J. M., Jr.; Booker, S. J. “Efficient Delivery of Long-Chain Fatty Aldehydes from the Nostoc punctiforme Acyl Acyl Carrier Protein Reductase to Its Cognate Aldehyde-Deformylating Oxygenase” Biochemistry 2015, 54, 1006-1015. [link to publisher’s website]
2014
Chang, W.-c.; Guo, Y.; Wang, C.; Butch. S. E.; Rosenzweig, A. C.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr. “Mechanism of the C5 Stereoinversion Reaction in the Biosynthesis of Carbapenem Antibiotics” Science 2014, 343, 1140-1144. [link to publisher’s website]
Matthews, M. L.; Chang, W.-c.; Layne, A. P.; Miles, L. A.; Krebs, C.; Bollinger, J. M., Jr. “Direct Nitration and Azidation of Aliphatic Carbons by an Iron-Dependent Halogenase” Nature Chem. Biol. 2014, 10, 209-215. [link to publisher’s website]
2013
Kwak, Y.; Jiang, W.; Dassama, L. M. K.; Park, K.; Bell, C. B., III.; Liu, L. V.; Wong, S. D.; Saito, M.; Kobayashi, Y.; Kitao, S.; Seto, M.; Yoda, Y.; Alp, E. E.; Zhao, J.; Bollinger, J. M., Jr.; Krebs, C.: Solomon, E. I. “Geometric and Electronic Structure of the Mn(IV)Fe(III) Cofactor in Class Ic Ribonucleotide Reductase: Correlation to the Class Ia Binuclear Non-Heme Iron Enzyme” J. Am. Chem. Soc. 2013, 135, 17573−17584. [link to publisher’s website]
Dassama, L. M. K.; Silakov, A.; Krest, C. M.; Calixto, J. C.; Krebs, C.; Bollinger, J. M., Jr.; Green, M. T. “A 2.8 Å Fe−Fe Separation in the Fe2III/IV Intermediate, X, from Escherichia coli Ribonucleotide Reductase” J. Am. Chem. Soc. 2013, 135, 16758−16761. [link to publisher’s website]
Anton, B. P.; Chang, Y.C.; Brown, P.; Choi, H. P.; Faller, L. L.; Guleria, J.; Hu, Z.; Klitgord, N.; Levy-Moonshine, A.; Maksad, A.; Mazumdar, V.; McGettrick, M.; Osmani, L.; Pokrzywa, R.; Rachlin, J.; Swaminathan, R.; Allen, B.; Housman, G.; Monahan, C.; Rochussen, K.; Tao, K.; Bhagwat, A. S.; Brenner, S. E.; Columbus, L.; de Cre´cy-Lagard, V.; Ferguson, D.; Fomenkov, A.; Gadda, G.; Morgan, R. D.; Osterman, A. L.; Rodionov, D. A.; Rodionova, I. A.; Rudd, K. E.; Soll, D.; Spain, J.; Xu, S. Y.; Bateman, A.; Blumenthal, R. M.; Bollinger, J. M., Jr.; Chang, W. S.; Ferrer, M.; Friedberg, I.; Galperin, M. Y.; Gobeill, J.; Haft, D.; Hunt, J.; Karp, P.; Klimke, W.; Krebs, C.; Macelis, D.; Madupu, R.; Martin, M. J.; Miller, J. H.; O’Donovan, C.; Palsson, B.; Ruch, P.; Setterdahl, A.; Sutton, G.; Tate, J.; Yakunin, A.; Tchigvintsev, D.; Plata, G.; Hu, J.; Greiner, R.; Horn, D.; Sjölander, K.; Salzberg, S. L.; Vitkup, D.; Letovsky, S.; Segre, D.; DeLisi, C.; Roberts, R. J.; Steffen, M.; Kasif, S. “The COMBREX Project: Design, Methodology, and Initial Results” PLOS Biology 2013, 11(8), e1001638. [link to publisher’s website]
Snyder, R. A.; Bell, C. B.; Diao, Y.; Krebs, C.; Bollinger, J. M., Jr.; Solomon, E. I. “Circular Dichroism, Magnetic Circular Dichroism, and Variable Temperature Variable Field Magnetic Circular Dichroism Studies of Biferrous and Mixed-Valent myo-Inositol Oxygenase: Insights into Substrate Activation of O2 Reactivity” J. Am. Chem. Soc. 2013, 135, 15851-15863. [link to publisher’s website]
Wang, C.; Chang, W.-c.; Guo, Y.; Huang, H.; Peck, S. C.; Pandelia, M.-E.; Lin, G. M.; Liu, H. W.; Krebs, C.; Bollinger, J. M., Jr. “Evidence that the Fosfomycin-Producing Epoxidase, HppE, Is a Non–Heme-Iron Peroxidase” Science 2013, 342, 991-995. [link to publisher’s website]
Wong, S. D.; Srnec, M.; Matthews, M. L.; Liu, L. V.; Kwak, Y.; Park, K.; Bell, C. B.; Alp, E. E.; Zhao, J.; Yoda, Y.; Kitao, S.; Seto, M.; Krebs, C.; Bollinger, J. M., Jr.; Solomon, E. I. “Elucidation of the Fe(IV)=O Intermediate in the Catalytic Cycle of the Halogenase SyrB2” Nature 2013, 499, 320-324. [link to publisher’s website]
Wörsdörfer, B.; Lingaraju, M.; Yennawar, N. H.; Boal, A. K.; Krebs, C.; Bollinger, J. M., Jr.; Pandelia, M.-E. “Organophosphonate-Degrading PhnZ Reveals an Emerging Family of HD Domain Mixed-Valent Diiron Oxygenases” Proc. Natl. Acad. Sci., U.S.A., 2013, 110, 18874-18879. [link to publisher’s website]
Dassama, L. M. K.; Krebs, C.; Bollinger, J. M., Jr.; Rosenzweig, A. C.; Boal, A. K. “Structural Basis for Assembly of the MnIV/FeIII Cofactor in the Class Ic Ribonucleotide Reductase from Chlamydia trachomatis” Biochemistry 2013, 52, 6424−6436. [link to publisher’s website]
Pandelia, M.-E.; Li, N.; Nørgaard, H.; Warui, D. M.; Rajakovich, L. J.; Chang, W.-c.; Booker, S. J.; Krebs, C.; Bollinger, J. M., Jr. “Substrate-Triggered Addition of Dioxygen to the Diferrous Cofactor of Aldehyde-Deformylating Oxygenase to Form a Diferric-Peroxide Intermediate” J. Am. Chem. Soc. 2013, 135, 15801−15812. [link to publisher’s website]
Wörsdörfer, B.; Conner, D. A.; Yokoyama, K.; Livada, J.; Seyedsayamdost, M.; Jiang, W.; Stubbe, J.; Bollinger, J. M., Jr.; Krebs, C. “Function of the Diiron Cluster of Escherichia coli Class Ia Ribonucleotide Reductase in Proton-Coupled Electron Transfer” J. Am. Chem. Soc. 2013, 135, 8585−8593. [link to publisher’s website]
Krebs, C.; Dassama, L. M. K.; Matthews, M. L.; Jiang, W.; Price, J. C.; Korboukh, V.; Li, N.; Bollinger, J. M., Jr. “Novel Approaches for the Accumulation of Oxygenated Intermediates to Multi-Millimolar Concentrations” Coord. Chem. Rev. 2013, 257, 234-243. [link to publisher’s website]
2012
Dassama, L. M. K.; Jiang, W.; Varano, P. T.; Pandelia, M.-E.; Conner, D. A.; Xie, J. J.; Bollinger J. M., Jr.; Krebs, C. “Radical-Translocation Intermediates and Hurdling of Pathway Defects in “Super-oxidized” (MnIV/FeIV) Chlamydia trachomatis Ribonucleotide Reductase” J. Am. Chem. Soc. 2012, 134, 20498-20506. [link to publisher’s website]
Li, N.; Chang, W.-c.; Warui, D. M.; Booker, S. J.; Krebs, C.; Bollinger, J. M., Jr. “Evidence for Only Oxygenative Cleavage of Aldehydes to Alk(a/e)nes and Formate by Cyanobacterial Aldehyde Decarbonylase” Biochemistry 2012, 51, 7908-7916. [link to publisher’s website]
Ye, S.; Riplinger, C.; Hansen, A.; Krebs, C., Bollinger, J. M., Jr., Neese, F. “Electronic Structure Analysis of the Oxygen-Activation Mechanism by Fe(II)- and alpha-Ketoglutarate (alpha KG)-Dependent Dioxygenases” Chem. – Eur. J. 2012, 18, 6555-6567. [link to publisher’s website]
Dassama, L. M. K.; Yosca, T. H.; Conner, D. A.; Lee, M.; Blanc, B.; Streit, B. R.; Green, M. T.; DuBois, J. L.; Krebs, C., Bollinger, J. M., Jr. “O2-Evolving Chlorite Dismutase as a Tool to Study O2-Utilizing Enzymes” Biochemistry 2012, 51, 1607-1616. [link to publisher’s website]
Dassama, L. M. K.; Boal, A. K., Krebs, C.; Rosenzweig, A. C.; Bollinger, J. M., Jr. “Evidence that the β Subunit of Chlamydia trachomatis Ribonucleotide Reductase is Active with the Manganese Ion of its Manganese(IV)/Iron(III) Cofactor in Site 1” J. Am. Chem. Soc. 2012, 134, 2520-2523. [link to publisher’s website]
2011
Li, N.; Nørgaard, H.; Warui, D. M.; Booker, S. J.; Krebs, C., Bollinger, J. M., Jr. “Conversion of Fatty Aldehydes to Alka(e)nes and Formate by a Cyanobacterial Aldehyde Decarbonylase: Cryptic Redox by an Unusual Di-Metal Oxygenase” J. Am. Chem. Soc. 2011, 133, 6158-6161. [link to publisher’s website]
Krebs, C., Bollinger, J. M., Jr.; Booker, S. J. “Cyanobacterial Alkane Biosynthesis Further Expands the Catalytic Repertoire of the Ferritin-Like “Di-Iron-Carboxylate” Proteins” Curr. Opin. Chem. Biol. 2011, 15, 291-303. [link to publisher’s website]
Warui, D. M.; Li, N.; Nørgaard, H.; Krebs, C., Bollinger, J. M., Jr.; Booker, S. J. “Detection of Formate, Rather than Carbon Monoxide, as the Stoichiometric Co-Product in Conversion of Fatty Aldehydes to Alkanes by a Cyanobacterial Aldehyde Decarbonylase” J. Am. Chem. Soc. 2011, 133, 3316-3319. [link to publisher’s website]
Panay, A. J.; Lee, M.; Krebs C.; Bollinger, J. M., Jr.; Fitzpatrick, P. F. “Evidence for a High Spin Fe(IV) Species in the Catalytic Cycle of a Bacterial Phenylalanine Hydroxylase” Biochemistry 2011, 50, 1928-1933. [link to publisher’s website]
2010
van der Donk, W. A.; Krebs, C.; Bollinger, J. M., Jr. “Substrate Activation by Iron Superoxo Intermediates” Curr. Opin. Struct. Biol., 2010, 20, 673–683. [link to publisher’s website]
Li, N.; Korneeva Korboukh, V.; Krebs, C.; Bollinger, J. M., Jr. “Four-Electron Oxidation of p-Hydroxylaminobenzoate to p-Nitrobenzoate by a Peroxodiferric Complex in AurF from Streptomyces thioluteus” Proc. Natl. Acad. Sci., U.S.A. 2010, 107, 15722–15727. [link to publisher’s website]
Flashman, E.; Hoffart, L. M.; Hamed, R. B.; Bollinger, J. M., Jr.; Krebs, C.; Schofield, C. J. “Evidence for the Slow Reaction of Hypoxia-Inducible Factor Prolyl Hydroxylase 2 with Oxygen” FEBS Journal 2010, 277, 4089–4099. [link to publisher’s website]
Jiang, W.; Xie, J.; Varano, P. T.; Krebs, C.; Bollinger, J. M., Jr. “Two Distinct Mechanisms of Inactivation of the Class Ic Ribonucleotide Reductase from Chlamydia trachomatis by Hydroxyurea: Implications for the Protein Gating of Inter-subunit Electron Transfer” Biochemistry 2010, 49, 5340–5349. [link to publisher’s website]
Ye, S.; Price, J. C.; Barr, E. W.; Green, M. T.; Bollinger, J. M., Jr.; Krebs, C.; Neese, F. “Cryoreduction of the NO-Adduct of Taurine:α-Ketoglutarate Dioxygenase (TauD) Yields an Elusive {FeNO}8 Species” J. Am. Chem. Soc. 2010, 132, 4739–4751. [link to publisher’s website]
2009
Bollinger, J. M., Jr.; Krebs, C. “Freeze-quench 57Fe- Mössbauer Spectroscopy: Trapping Reactive Intermediates” Photosynthesis Research, 2009, 102, 295-304. [link to publisher’s website]
Korneeva Korboukh, V.; Li, N.; Barr, E. W.; Bollinger, J. M., Jr.; Krebs, C. “A Long-Lived, Substrate-Hydroxylating Peroxodiiron(III/III) Intermediate in the Amine Oxygenase, AurF, from Streptomyces thioluteus” J. Am. Chem. Soc. 2009, 131, 13608-13609. [link to publisher’s website]
Matthews, M. L.; Neumann, C. S.; Miles, L. A.; Grove, T. L.; Booker, S. J.; Krebs, C.; Walsh, C. T.; Bollinger, J. M., Jr. “Substrate Positioning Controls the Partition between Halogenation and Hydroxylation in the Aliphatic Halogenase, SyrB2” Proc. Natl. Acad. Sci., U.S.A. 2009, 106, 17723-17728. [link to publisher’s website]
Matthews, M. L.; Krest, C. M.; Barr, E. W.; Vaillancourt, F. H.; Walsh, C. T.; Green, M. T.; Krebs, C.; Bollinger, J. M., Jr. “Substrate-Triggered Formation and Remarkable Stability of the C- H Bond-Cleaving Chloroferryl Intermediate in the Aliphatic Halogenase, SyrB2” Biochemistry 2009, 48, 4331-4343. [link to publisher’s website]
Bollinger, J. M., Jr.; Diao, Y.; Matthews, M. L.; Xing, G.; Krebs, C. “myo-Inositol Oxygenase: A Radical New Pathway for O2 and C-H Activation at a Nonheme Diiron Cluster” Dalton Transactions 2009, 905-914. [link to publisher’s website]
2008
Bollinger, J. M., Jr.; Jiang, W.; Green, M. T.; Krebs, C. “The Manganese(IV)/Iron(III) Cofactor of Chlamydia trachomatis Ribonucleotide Reductase: Structure, Assembly, Radical Initiation, and Evolution” Curr. Opin. Struct. Biol. 2008, 18, 650-657. [link to publisher’s website]
Jiang, W.; Yun, D.; Saleh, L.; Bollinger, J. M., Jr.; Krebs, C. “Formation and Function of the Manganese(IV)/Iron(III) Cofactor in Chlamydia trachomatis Ribonucleotide Reductase” Biochemistry 2008, 47, 13736-13744. [link to publisher’s website]
Younker, J. M.; Krest, C. M.; Jiang, W.; Krebs, C.; Bollinger, J. M., Jr.; Green, M. T. “Structural Analysis of the Mn(IV)/Fe(III) Cofactor of Chlamydia trachomatis Ribonucleotide Reductase by Extended X-ray Absorption Fine Structure Spectroscopy and Density Functional Theory Calculations” J. Am. Chem. Soc. 2008, 130, 15022-150271. [link to publisher’s website]
Jiang, W.; Saleh, L.; Barr, E. W.; Xie, J.; Maslak-Gardner, M.; Krebs, C.; Bollinger, J. M., Jr. “Branched Activation- and Catalysis-Specific Pathways for Electron Relay to the Manganese/Iron Cofactor in Ribonucleotide Reductase from Chlamydia trachomatis” Biochemistry 2008, 47, 8477-8484. [link to publisher’s website]
Jiang, W.; Xie, J.; Nørgaard, H.; Bollinger, J. M., Jr.; Krebs, C. “Rapid and Quantitative Activation of Chlamydia trachomatis Ribonucleotide Reductase by Hydrogen Peroxide” Biochemistry 2008, 47, 4477-4483. [link to publisher’s website]
2007
Neidig, M. L.; Brown, C. D.; Light, K.; Galonić Fujimori, D.; Nolan, E. M.; Price, J. C.; Barr, E. W.; Bollinger, J. M., Jr.; Krebs, C.; Walsh, C. T.; Solomon, E. I. “CD and MCD of CytC3 and Taurine Dioxygenase: Role of the Facial Triad in α-KG-dependent Dioxygenases” J. Am. Chem. Soc. 2007, 129, 14224-14231. [link to publisher’s website]
Galonić Fujimori, D.; Barr, E. W.; Matthews, M. L.; Koch, G. M.; Yonce, J. R.; Walsh, C. T., Bollinger, J. M., Jr.; Krebs, C.; Riggs-Gelasco, P. J. “Spectroscopic Evidence for a High-Spin Fe(IV)-Oxo Intermediate in the α-Ketoglutarate-Dependent Halogenase CytC3 from Streptomyces” J. Am. Chem. Soc. 2007, 129, 13408-13409. [link to publisher’s website]
Eser, B.; Barr, E. W.; Frantom, P. A.; Saleh, L.; Bollinger, J. M., Jr.; Krebs, C.; Fitzpatrick, P. F. “Direct Spectroscopic Evidence for a High-Spin Fe(IV) Intermediate in Tyrosine Hydroxylase” J. Am. Chem. Soc. 2007, 129, 11334-11335. [link to publisher’s website]
Krebs, C.; Matthews, M. L.; Jiang, W.; Bollinger, J. M., Jr. “AurF from Streptomyces thioluteus and a Possible New Family of Manganese/Iron Oxygenases” Biochemistry 2007, 46, 10413-10418. [link to publisher’s website]
Jiang, W.; Hoffart, L. M.; Krebs, C.; Bollinger, J. M., Jr. “Direct Detection of a Mn(IV)/Fe(IV) Intermediate in Assembly of the Mn(IV)/Fe(III) Cofactor of Chlamydia trachomatis Ribonucleotide Reductase” Biochemistry 2007, 46, 8709-8716. [link to publisher’s website]
Krebs, C.; Galonić Fujimori, D.; Walsh, C. T.; Bollinger, J. M., Jr. “Non-heme Fe(IV)-Oxo Intermediates” Acc. Chem. Res. 2007, 40, 484-492. [link to publisher’s website]
Jiang, W.; Bollinger, J. M., Jr.; Krebs, C. “The Active Form of Chlamydia trachomatis Ribonucleotide Reductase R2 Protein Contains a Heterodinuclear Mn(IV)/Fe(III) Cluster with S = 1 Ground State” J. Am. Chem. Soc. 2007, 129, 7504-7505. [link to publisher’s website]
Jiang, W.; Yun, D.; Saleh, L.; Barr, E. W.; Xing, G.; Hoffart, L. M.; Maslak, M.-A.; Krebs, C.; Bollinger, J. M., Jr. “A Stable Manganese(IV)/Iron(III) Cofactor Initiates Substrate Radical Production in Chlamydia trachomatis Ribonucleotide Reductase” Science, 2007, 316, 1188-1191. [link to publisher’s website]
Sinnecker, S.; Svensen, N.; Barr, E. W.; Ye, S.; Bollinger, J. M., Jr.; Neese, F.; Krebs, C. “Spectroscopic and Computational Evaluation of the Structure of the High-Spin Fe(IV)-Oxo Intermediates in Taurine:alpha-Ketoglutarate Dioxygenase from Escherichia coli and its His99Ala Ligand Variant” J. Am. Chem. Soc. 2007, 129, 6168-6179. [link to publisher’s website]
Bollinger, J. M., Jr.; Krebs, C. “Enzymatic C-H Activation by Metal-Superoxo Intermediates” Curr. Opin. Chem. Biol. 2007, 11, 151-158. [link to publisher’s website]
Galonić, D. P.; Barr, E. W.; Walsh, C. T.; Bollinger, J. M., Jr.; Krebs, C. “Two Interconverting High-Spin Fe(IV) Intermediates in Aliphatic Chlorination by the Fe(II)- and α-Ketoglutarate-Dependent Halogenase CytC3” Nature Chem. Biol. 2007, 3, 113-116. [link to publisher’s website]
2006
Hoffart, L. M.; Barr, E. W.; Guyer, R. B.; Bollinger, J. M., Jr.; Krebs, C. “Direct Spectroscopic Detection of a C-H-Cleaving High-Spin Fe(IV) Complex in a Prolyl-4-Hydroxylase” Proc. Natl. Acad. Sci., U.S.A. 2006, 103, 14738-14743. [link to publisher’s website]
Kim, S. H.; Xing, G.; Bollinger, J. M. Jr.; Krebs, C.; Hoffman, B. M. “Demonstration by 2H ENDOR Spectroscopy that myo-Inositol Binds via an Alkoxide Bridge to the Mixed-Valent Diiron Center of myo-Inositol Oxygenase” J. Am. Chem. Soc. 2006, 128, 10374-10375. [link to publisher’s website]
Xing, G.; Diao, Y.; Hoffart, L. M.; Barr, E. W.; Prabhu, K. S.; Arner, R. J.; Reddy, C. C.; Krebs, C.; Bollinger, J. M., Jr. “Evidence for C-H Cleavage by an Iron-Superoxide Complex in the Glycol Cleavage Reaction Catalyzed by myo-Inositol Oxygenase” Proc. Natl. Acad. Sci., U.S.A. 2006, 103, 6130-6135. [link to publisher’s website]
Xing, G.; Barr, E. W.; Diao, Y.; Hoffart, L. M.; Prabhu, K. S.; Arner, R. J.; Reddy, C. C.; Krebs, C.; Bollinger, J. M., Jr. “Oxygen Activation by a Mixed-Valent, Diiron(II/III) Cluster in the Glycol Cleavage Reaction Catalyzed by myo-Inositol Oxygenase” Biochemistry 2006, 45, 5402-5412. [link to publisher’s website]
Xing, G.; Hoffart, L. M.; Diao, Y.; Prabhu, K. S.; Arner, R. J.; Reddy, C. C.; Krebs, C.; Bollinger, J. M., Jr. “A Coupled Dinuclear Iron Cluster that Is Perturbed by Substrate Binding in myo-Inositol Oxygenase” Biochemistry 2006, 45, 5393-5401. [link to publisher’s website]
Bollinger, J. M., Jr.; Krebs, C. “Stalking Intermediates in Oxygen Activation by Iron Enzymes: Motivation and Method” J. Inorg. Biochem. 2006, 100, 586-605. [link to publisher’s website]
2005
Bollinger, J. M., Jr.; Price, J. C.; Hoffart, L. M.; Barr, E. W.; Krebs, C. “Mechanism of Oxygen Activation by Taurine:a-Ketoglutarate Dioxygenase (TauD) from Escherichia coli” Eur. J. Inorg. Chem. 2005, 2005, 4245-4254. [link to publisher’s website]
Price, J. C.; Barr, E. W.; Hoffart, L. M.; Krebs, C.; Bollinger, J. M., Jr. “Kinetic Dissection of the Catalytic Mechanism of Taurine:a-Ketoglutarate Dioxygenase (TauD) from Escherichia coli” Biochemistry 2005, 44, 8138-8147. [link to publisher’s website]
Krebs, C.; Price, J. C.; Baldwin, J.; Saleh, L.; Green, M. T.; Bollinger, J. M., Jr. “Rapid Freeze-Quench 57Fe Mössbauer Spectroscopy: Monitoring Changes of an Iron-Containing Active Site during a Biochemical Reaction” Inorg. Chem. 2005, 44, 742-757. [link to publisher’s website]
2004
Riggs-Gelasco, P. J.; Price, J. C.; Guyer, R. B.; Brehm, J. H.; Barr, E. W.; Bollinger, J. M., Jr.; Krebs, C. “EXAFS Spectroscopic Evidence for an Fe=O Unit in the Fe(IV) Intermediate Observed during Oxygen Activation by Taurine:a-Ketoglutarate Dioxygenase” J. Am. Chem. Soc. 2004, 126, 8108-8109. [link to publisher’s website]
2003
Price, J. C.; Barr, E. W.; Glass, T. E.; Krebs, C.; Bollinger, J. M., Jr. “Evidence for Hydrogen Abstraction from C1 of Taurine by the High-Spin Fe(IV) Intermediate Detected during Oxygen Activation by Taurine:a-Ketoglutarate Dioxygenase (TauD)” J. Am. Chem. Soc. 2003, 125, 13008-13009. [link to publisher’s website]
Price, J. C.; Barr, E. W.; Tirupati, B.; Bollinger, J. M., Jr.; Krebs, C. “The First Direct Characterization of a High-Valent Iron Intermediate in the Reaction of an a-Ketoglutarate-Dependent Dioxygenase: A High-Spin Fe(IV) Complex in Taurine/a-Ketoglutarate Dioxygenase (TauD) from Escherichia coli” Biochemistry 2003, 42, 7497-7508. [link to publisher’s website]