Publications

Independent Lab (Hedglin Lab Members in BOLD, Undergraduate Hedglin Lab Members are also Underlined)

  1. Norris J.L., Hedglin M. Direct, ensemble FRET approaches to monitor transient state kinetics of human DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis. Methods in Enzymology: Chemical and Genomic Methods in Nucleic Acid Biology. 2024 In Press, Corrected Proof, Available Online 08/28/2024. https://doi.org/10.1016/bs.mie.2024.08.002. Click on Share Link before 10/17/2024 for access.
  1. Norris J.L., Rogers L.O., Pytko K.G., Dannenberg R.L., Perreault S., Kaushik V., Kuppa S., Antony E., Hedglin M. Replication protein A dynamically re-organizes on primer/template junctions to permit DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis. Nucleic Acids Res. 2024 Jul 22;52(13):7650-7664. https://doi.org/10.1093/nar/gkae475. PMID: 38842913; PMCID: PMC11260492.
  1. Muoio D., Laspata N., Dannenberg R.L., Curry C., Darkoa-Larbi S., Hedglin M., Uttam S., Fouquerel E. PARP2 promotes Break Induced Replication-mediated telomere fragility in response to replication stress.  Nature Communications 15, 2857 (2024). https://doi.org/10.1038/s41467-024-47222-7.
  1. Stroik S. Carvajal-Garcia J., Gupta D., Edwards A., Luthman A., Wyatt D.W., Dannenberg R.L., Feng W., Kunkel T.A., Gupta G.P., Hedglin M., Wood R., Doublie S., Rothenberg E., Ramsden D.A.  Stepwise requirements for polymerases δ and θ in theta-mediated end joining. Nature (2023). https://doi.org/10.1038/s41586-023-06729-7
  1. Pytko K.G., Dannenberg R.L., Eckert K.A., Hedglin M. Replication of [AT/TA]25 Microsatellite Sequences by Human DNA Polymerase δ Holoenzymes Is Dependent on dNTP and RPA Levels. Biochemistry. 2024 Apr 16;63(8):969-983. https://pubs.acs.org/doi/10.1021/acs.biochem.4c00006. Epub 2024 Mar 26. PMID: 38623046.

  1. Hoitsma N.M., Norris J.L., Khoang T.H., Kaushik V., Antony E.,  Hedglin M., Freudenthal B.D. Mechanistic insight into AP-Endonuclease 1 cleavage of abasic sites at stalled replication forks. Nucleic Acids Research, 2023; gkad481, https://doi.org/10.1093/nar/gkad481
  1. Dannenberg, R.L., Cardina, J.A., Pytko, K.G., and Hedglin, M. Tracking of progressing human DNA polymerase δ reveals distributions of DNA lesion bypass activities. Nucleic Acids Research, 2022, gkac745, https://doi.org/10.1093/nar/gkac745
  1. Li, M., Sengupta, B., Benkovic, S.J., Lee, T.H., and Hedglin, M. PCNA monoubiquitinationis regulated by diffusion of Rad6/Rad18 complexes along RPA filaments. Biochemistry, 2020 Nov 27. doi: 10.1021/acs.biochem.0c00849. Online ahead of print.

Abstract Image

  1. Gaubitz, C., Liu, X., Magrino, J., Stone, N.P., Landeck, J., Hedglin, M, and Kelch, B.A.Structure of the human  clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch. PNAS, Sep 2020, 202007437; DOI: 10.1073/pnas.2007437117. https://www.pnas.org/content/early/2020/09/08/2007437117
  1. Shin, Y., Hedglin, M., and Murakami, K.S. Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase. Nucleic Acids Research, 2020, gkz1221, https://doi.org/10.1093/nar/gkz1221
  1. Li M., Larsen L., and Hedglin, M. Rad6/Rad18 competes with DNA polymerases η and δ for PCNA encircling DNA. Biochemistry, 2019, Dec 30. doi: 10.1021/acs.biochem.9b00938. [Epub ahead of print]. https://pubs.acs.org/doi/10.1021/acs.biochem.9b00938

  1. *Hedglin, M., Aitha M., and Benkovic, S.J. Replication protein A dynamically regulates monoubiquitination of proliferating cell nuclear antigen. J Biol Chem, 2019, 294, 5157-5168. http://www.jbc.org/content/294/13/5157.long

         * = Corresponding Author

Training:

  1. Hedglin, M., Aitha M., and Benkovic, S.J. Monitoring the Retention of Human PCNA at Primer/Template junctions by Proteins that Bind Single-stranded DNA. Biochemistry, 2017, 56, 3415 – 3421. https://pubs.acs.org/doi/10.1021/acs.biochem.7b00386

         

  1. Hedglin, M. and Benkovic, S.J. Eukaryotic Translesion DNA Synthesis on the Leading and Lagging Strands: Unique Detours Around the Same Obstacle. 2017, ACS Chemical Reviews, 117, 7857 – 7877. https://pubs.acs.org/doi/10.1021/acs.chemrev.7b00046

  1. Hedglin, M. and Benkovic, S.J. Replication protein A prohibits diffusion of the PCNA sliding clamp along single-stranded DNA. Biochemistry, 2017, 56, 1824 – 1835. https://pubs.acs.org/doi/10.1021/acs.biochem.6b01213

  1. Hedglin, M., Pandey, B., and Benkovic, S.J. Characterization of human translesion DNA synthesis across a UV-induced DNA lesion. 2016, eLife 5, e19788. https://elifesciences.org/articles/19788
  1. Hedglin, M., Pandey, P., and Benkovic, S.J. Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis. 2016, Proc Natl Acad Sci U S A, 113, E1777 – E1786. https://www.pnas.org/content/113/13/E1777.long
  1. Hedglin, M., Zhang, Y., and O’Brien, P.J. Probing the DNA structural requirements for facilitated diffusion. Biochemistry, 2015, 54, 557-566. https://pubs.acs.org/doi/10.1021/bi5013707

  1. Hedglin, M., and Benkovic, S.J. Regulation of Rad6/Rad18 Activity During DNA Damage Tolerance. Annu Rev Biophys, 2015, 44, 207-228. https://www.annualreviews.org/doi/full/10.1146/annurev-biophys-060414-033841?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed
  1. Hedglin, M., Zhang, Y., and O’Brien, P.J. Isolating contributions from intersegmental transfer to DNA searching by alkyladenine DNA glycosylase. J Biol Chem, 2013, 288, 24550-24559. http://www.jbc.org/content/288/34/24550.long
  1. Hedglin, M., Perumal, S.K., Hu, Z., and Benkovic, S. Stepwise assembly of the human replicative polymerase holoenzyme. eLife, 2013, 2, e00278. https://elifesciences.org/articles/00278
  1. Hedglin, M., Kumar, R., and Benkovic, S.J. Replication clamps and clamp loaders. Cold Spring Harb Perspect Biol, 2013, 5, a010165. https://cshperspectives.cshlp.org/content/5/4/a010165.long
  1. Hedglin, M., and O’Brien, P.J. Hopping enables a DNA repair glycosylase to search both strands and bypass a bound protein. ACS Chem Biol, 2010, 5, 427-436. https://pubs.acs.org/doi/abs/10.1021/cb1000185

  1. Hedglin, M., and O’Brien, P.J. Human alkyladenine DNA glycosylase employs a processive search for DNA damage. Biochemistry, 2008, 47, 11434-11445. https://pubs.acs.org/doi/10.1021/bi801046y

  1. Krennhrubec, K., Marshall, B.L., Hedglin, M., Verdin, E., and Ulrich, S.M. Design and evaluation of ‘Linkerless’ hydroxamic acids as selective HDAC8 inhibitors. Bioorg Med Chem Lett, 2007, 17, 2874-2878. https://www.sciencedirect.com/science/article/pii/S0960894X07002612?via%3Dihub