Spectrometry:

Electron Paramagnetic Resonance Spectroscopy

– Bruker X-band E580 pulse EPR spectrometer augmented with a home-build Q-band extension

This equipment is capable of performing X- and Q-band ENDOR, ESEEM, HYSCORE, DEER and other advanced pulse EPR measurements at temperatures from 4K to 300K

Fourier Transform Infrared Spectroscopy

– Nicolet iS 50 FT-IR Spectrometer

Currently this spectrometer is set up for transmission measurements using a home-build IR cell. We are planning to add spectroelectrochemical capabilities and the ability to perform transmission measurements at cryogenic temperatures.

UV/Vis

– Agilent Cary 60

Gas chromotography

– Agilent 7890A with Thermal  Conductivity  Detector (TCD)

Anaerobic protein sample preparations:

Anaerobic chamber

– Coy Glove Box

Used for anaerobic protein isolation and purification and anaerobic sample preparation for spectroscopic characterization.

Equipped with home-built two-line gas manifold with flow controls for controlled gas treatment

Rotating electrode electrochemical setup based on Pine Research CRP rotator and operated by Princeton Applied Research VersaSTAT3 potentiostat for protein film voltammetry experiments on proteins.

 

FPLC

– GE Akta Start

Used for sample purification under anaerobic conditions.

Basic laboratory equipment:

– Thermo Scientific Sorvall Lynx 4000 centrifuge

– Deli cabinet, -20C and -80C fridges

–  New Brunswick Escella E25 temperature controlled shaker

– Fume hood with a Schlenk line

– Labnet Multygene Optimax thermocycler

– Pink pipettes