Missed events in biexpoential fits
Missed events in biexpoential fits

Author: William Hancock Problem:  Very often we measure distributions of times, run lengths, or other quantities, we plot the distribution, and we fit it with an exponential or biexponential.  Also,

Binding Densities in Flow Cells
Binding Densities in Flow Cells

Author: William Hancock Q:  What concentration of tubulin is needed to create a full monolayer of tubulin on the surface of a flow cell? A:  Tubulin is 8 nm x

Calculating Ionic Strength of Buffers
Calculating Ionic Strength of Buffers

Author: William Hancock For electrostatic interactions of proteins, the ionic strength is a major determinant, and so we want to be able to compare buffers of different ionic strengths.  The

How much protein do I lose to the side of my tube?
How much protein do I lose to the side of my tube?

Author: William Hancock Say you have about 30 ul in an Eppendorf tube.  You can lose protein by protein adsorbing to the side of the tube.  How much might you

A Guide to Keeping a Good Laboratory Notebook
A Guide to Keeping a Good Laboratory Notebook

Author: William Hancock General Comments: The purpose of a lab notebook is to keep a record of the experiments you have done so that you can refer back to them