Illumina NGS read number and length

Since we often get asked, here is a quick reference for MiSeq (we have two), NextSeq (UP Core has one), and NovaSeq (we have one).

flow cell type	read#	read length
MiSeq Reagent Kit v2	12-15 million	1 × 36 bp	2 × 25 bp	2 × 150 bp	2 × 250 bp
MiSeq Reagent Kit v3	22–25 million	2 × 75 bp	2 × 300 bp
MiSeq Reagent Kit v2 Micro	4 million	2 × 150 bp
MiSeq Reagent Kit v2 Nano	1 million	2 × 150 bp	2 × 250 bp
NextSeq 550 System Mid-Output Kit*	130 Million	2 × 75 bp	2 × 150 bp
NextSeq 550 System High-Output Kit*	400 Million	1 × 75 bp	2 × 75 bp	2 × 150 bp
NovaSeq SP	650–800 Million	2 × 50 bp	2 × 100 bp	2 × 150 bp	2 x 250 bp
NovaSeq S1	1.3–1.6 Billion	2 × 50 bp	2 × 100 bp	2 × 150 bp
NovaSeq S2	3.3 B–4.1 Billion	2 × 50 bp	2 × 100 bp	2 × 150 bp
NovaSeq S4	8-10 Billion	1 × 35 bp	2 × 100 bp	2 × 150 bp
*: available at UP Genomics Core

Yuka Imamura Kawasawa